Today, one of the significant issues in the realm of religion is the investigation and analysis of the effective preaching role of seminary students in universities. This research delves into the preaching behaviors of seminaries in the recent century within universities, aiming to demonstrate the effectiveness of seminaries and religious students in promoting religious culture and preaching religion in universities. Using a descriptive-analytical method and library research, this study addresses the question of how the preaching role of seminaries in universities is perceived. The research proceeds in two steps: firstly, it elucidates the relationship between seminaries and universities during the Pahlavi era. Preaching behaviors of seminaries before the revolution were limited in discourse, with the presence of notable figures of clerics like Ayatollah Morteza Motahhari, the late Martyr Mofatteh, and Ayatollah Khamenei in universities. Secondly, it analyzes the relationship between seminaries and universities after the revolution. The preaching behaviors of seminaries in post-revolution era, expanded significantly within the revolutionary discourse space, marked by quantitative and qualitative growth, including the establishment of the “Leadership Institution in Universities” and dispatching seminary students to universities.